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KMID : 0861020070220020147
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Korea Journal of Herbology
2007 Volume.22 No. 2 p.147 ~ p.153
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ERK mediated suppressive effects of Sophora flavescens on Tnf alpha production in BV2 microglial cells
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Jung Kyung-Hee
Kim Soo-Chul
Han Mi-Young
Park Hye-Jeong
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Abstract
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Objectives : Sophora flavescens (SF) is widely used in traditional herbal medicine in Korea and is well recognized for its anti-inflammatory effect. However, its effect on Tumornecrosis factor alpha (Tnf) production in BV2 microglial cell is not yet known.
Methods : We investigated the effect of SF on the production and expression of Tnf, a well known inflammatory mediator, in lipopolysaccaride (LPS)-activated BV2 microglial cells.
Results : The LPS-induced Tnf production was markedly reduced by treatment with SF (50 {mu}g/ml). In reverse transcription polymerase chain reaction (RT-PCR) analysis, SF suppressed the LPS activated expression of Tnf mRNA. In addition, Western blot analysis confirmed that SF suppressed the expression of Tnf. Sophora flavescens also inhibited the LPS-induced phosphylation of extracellular signal-regulated kinases (ERK), which mediate the Tnfproduction signaling pathway whereas LPS-induced phosphylation of p38 mitogen activated protein kinase (p38 MAPK), and c-Jun NH2-terminal kinases (JNK) was not inhibited by SF, which implies that SF suppresses LPS-induced Tnf production via the ERK mediated pathway.
Conclusion : Taken together, these findings indicated that SF inhibits LPS-induce Tnf production, and that this inhibitory effect is mediated via the ERK pathway.
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KEYWORD
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Sophora flavescens, tumor necrosis factor alpha (Tnf), extracellular signal-regulated kinases (ERK), p38 mitogen activated protein kinase (p38 MAPK), c-Jun NH2-terminal kinases (JNK), inflammation
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